Mouse non-histone chromosomal protein HMG-14 cDNA sequence
نویسندگان
چکیده
منابع مشابه
Recombinant human chromosomal proteins HMG-14 and HMG-17.
Vectors for expressing human chromosomal proteins HMG-14 and HMG-17 in bacterial cultures under the control of the temperature-inducible lambda PL promoter have been constructed. The open reading frames of the cDNAs have been amplified by the polymerase chain reaction (PCR), utilizing amplimers containing desired restriction sites, thereby facilitating precise location of the initiation codon d...
متن کاملSingle copy gene for the chicken non-histone chromosomal protein HMG-17.
A chicken genomic library was screened with the human cDNA encoding the non-histone chromosomal protein HMG-17 and a 4565-base pair fragment containing the entire gene encoding this protein was isolated and characterized. Sequence analysis of the fragment revealed that from the start to end of transcription, the HMG-17 gene is 3293 base pairs long and is comprised of 6 exons ranging in size fro...
متن کاملTissue-specific Chromosomal Non-histone Protein Interactions
A group of chromosomal non-histone proteins with affinity for DNA (NP) was isolated from rat liver and Novikoff hepatoma. This fraction, which represents less than 5% of the total chromatin protein content, binds preferentially to unique, double-stranded sequences of fractionated homologous DNA. The interactions are strong at low ionic strength (K, = 6.7 x lOme M) and decrease with rising salt ...
متن کاملNucleotide sequence of a mouse cDNA encoding the nonhistone chromosomal high mobility group protein-1 (HMG1)
The high mobility group (HMG) proteins are the major nonhistone components of chromatin (1). Among these, the HMG1 and HMG2 proteins are the most abundant; however, their precise function remains undefined. There are some indications for a putative role of HMG 1/2 in chromatin assembly (2), DNA replication (3) or even as general transcription factors for RNA polymerase II (4). There is a consid...
متن کاملA human placental cDNA clone that encodes nonhistone chromosomal protein HMG-1.
From a human placental lambda gt11 cDNA library, we have isolated a cDNA clone that encodes the entire 215-residue amino acid sequence of HMG-1. Analysis of an internal sequence similarity suggests that the DNA-binding domains of HMG-1 are separated by a rather long and flexible linker segment. Southern blotting of DNA digested with BamHI indicated a highly variable number of genes (or pseudoge...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.17.5311